The method we apply in separating components of a mixture depends on the nature.
Physical methods like decantation and filtration separate some mixtures.
A mixture with two or more solutes in a solution is difficult to separate physically. The components of such a mixture can be separated through a medium using a solvent.
Components of a mixture in such a solution can be separated by chromatography.
Chromatography is a technique of separating components of a homogenous mixture whose solubility in a solvent differs.
A solvent that can dissolve the components in a mixture is added to the mixture on a medium such as chromatography paper or filter paper.
The component of the mixture that is more soluble in the solvent moves furthest from the baseline. The least soluble component moves the shortest distance from the spot of the mixture.
There are many types of chromatography. The common ones are:
- Liquid chromatography
- Paper chromatography
- Gas chromatography
- Ion exchange chromatography
- Column chromatography
- High-performance liquid chromatography
- Ultra-high performance liquid chromatography
The type of chromatography we apply in separating a mixture depends on the nature of the components.
Components with high boiling points are separated by gas chromatography while those with lower boiling points are separated by liquid chromatography. Colored pigments are separated by paper chromatography.
Here are five examples of mixtures separated by chromatography:
1. Colored Pigments in Ink
Colored ink components have different degrees of solubility in water, ethanol, or propanone.
When a filter paper with a spot of ink is put in ethanol, the color pigments spread out, covering different distances from the spot.
The line where the ink spot is put is the baseline, while the line through the furthest point the solvent reaches is the solvent front.
The filter paper is the stationary phase while ethanol is the mobile phase.
2. A mixture of amino acids
Amino acids dissolve in phenol to form colored products.
When a spot of the mixture is put on a chromatography paper and then dipped in phenol, the amino acids form different colored products with different degrees of solubility.
3. A mixture of different drugs
In forensics, gas chromatography is applied to detect banned substances or poisoning in a drug.
The drug suspected to contain banned substances is run through a gas chromatogram.
Each substance develops its unique mass spectrum in the gas chromatogram.
4. Separation of carbohydrates in a mixture
Carbohydrates in a mixture are separated by thin-layer chromatography (TLC).
The carbohydrate molecules are depolarized before moving through a layer of silica gel.
Different carbohydrates show different degrees of adsorption.
5. Separation of narcotic components
Heroin can be separated from other narcotics by high-performance liquid chromatography ( HPLC).
The mixture is added to propanol in the mobile phase and delivered to the column, which is the stationary phase.
The mixture is delivered at high pressure from the mobile phase to the column—different components in the mixture flow at different speeds.
Heroin has a low affinity for propanol and flows to the stationary phase faster.
Closing Thoughts
Chromatography is a simple technique of separating components in a mixture with a wide range of applications.
In forensics, it helps to detect consumption of banned substances; in sports, it helps detect doping cases; and in the pharmaceutical industry, it helps to detect the use of banned substances in drugs.
Chromatography requires a small sample for analysis and is applied to a wide range of mixtures.
It is done on simple and readily available apparatus and produces pure samples of components.
This makes chromatography the most popular and widely used method of separation.
